This study examined chardonnay, muscadine, concord, and ruby red grape seed oil and flour extracts for antioxidant and anti-proliferative properties. The extracts were tested for total phenolic content, DPPH• and ABTS•+ radical scavenging capacity and effect against HT-29 cancer cell proliferation.;All of the grape seed extracts exhibited ABTS•+ and DPPH• radical quenching activity. The flour extracts from chardonnay demonstrated the highest ABTS•+ radical quenching capacity of 186 mumoles trolox equivalents/g and DPPH• radical quenching activity of 30 mumoles trolox equivalents/g. All of the grape seed oil and flour extracts contained significant levels of phenolics. Chardonnay grape seed flour extract displayed the most prominent dose dependent anti-proliferative effect. Muscadine and ruby red, but not concord grape seed flour extracts, also exhibited anti-proliferative activities. The collected data advocate for the potential of grape seed extracts as dietary sources of anti-proliferative and antioxidant components.