Chitosan is a deacetylated product of chitin. Chitosan possesses positive charges, which give it the ability to bind negatively charged fats, proteins, molecules, etc. Chitosan has attained increasing interest as a biomaterial, due to its favorable properties including biocompatibility, biodegradability.;The current research involves using N-(R)-succinamic acid to modify chitosan utilizing amide bond formation by diimide coupling reaction. Formation of this amide bond enhances hydrogen bonding to the collagen backbone in turn increasing the efficacy of chitosan in its diverse applications. The effects of molecular chain length, hydrophobicity, and aromaticity on the modification were investigated by using different amount of six N-(R)-succinamic acids reacted with native chitosan in aqueous solutions at room temperature.;IR and NMR were used to determine the degree of deacetylation (DDA), an important parameter of chitosan that affects its physicochemical properties. The introduction of amide groups increased the hydroscopic nature of the samples. This additional moisture interfered with the quality of the IR spectra rendering IR ineffective for the determination of DDA. The NMR results showed the protons on the modified structure were seen at 2.8∼3.3 ppm and 1.2∼1.9 ppm. The integrations of protons on the deacetylated structure, the acetyl group, and the modified structure were compared to determine the DDA. The amine sites on the chitosan could be modified from 9% up to 23%. The results showed the efficiency of chitosan modification is dependent on the chemical nature of the modifying agent. An increase in the size and hydrophobic of modifying reagents results in more effective modification. Aromatic modifiers displayed better modification than aliphatic modifiers.;SEC was used to study the molecular hydrodynamic behavior. Chitosan was incubated under room temperature (20°C) at different time length. The hydrodynamic behavior was investigated by